Volume 21 Issue 2 2026

Pila globosa is found in freshwater ecosystems such as ponds, streams, rivers, lakes, rice fields, irrigation canals, and wetlands with low salinity. In this study, we aimed to analyze the change in the nutritional levels and histological changes of Pila globosa in changing pH levels. To study the pH tolerance, the snails were exposed to different pH (4, 5, 6, 8, 9, 10). At pH 4 and 10, snails showed 100% mortality rate within 24 hours. The snails treated with pH 5, 6, 8, and 9 survived throughout the experiment. These snails were kept in the treated water for about 72 hours (3 days). After that, biochemical and histological studies were performed for nutritional level. The foot and digestive gland were used for histological evaluation. The control group, having pH 7, showed a mean value of 0.133±0.003 gm/gm protein in foot tissue. The snails with pH 5, 6, 8, 9 showed a mean value of 0.122± 0.004, 0.127±0.003, 0.142±0.003 and 0.146±0.003 gm/gm of tissue protein, respectively. The histological analysis of the foot showed the changes in disruption of the epidermis and formation of large vacuoles. And the histological analysis of the digestive gland showed more K corpuscles in pH 5 and pH 9 in comparison with the other two treated groups and the control.